Regensburg 2010 – scientific programme
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BP: Fachverband Biologische Physik
BP 36: Posters: Tissue Dynamics, Charge Effects, and Anomalous Transport
BP 36.11: Poster
Thursday, March 25, 2010, 17:15–20:00, Poster B2
Non-ballistic and subdiffusive nanoparticle transport in living cells — •Marcus Otten1, Amitabha Nandi2, Delphine Arcizet1, Benjamin Lindner2, and Doris Heinrich1 — 1Ludwig-Maximilians-Universität and Center for NanoScience (CeNS), München, Germany — 2Max-Planck-Institut für Physik komplexer Systeme, Dresden, Germany
Intracellular transport of vesicles, macromolecules and organelles relies on ballistic and diffusive (including subdiffusive, Brownian and superdiffusive) motion. Local mean square displacement (MSD) analysis allows for the time-resolved separation of these two motion types and their respective motion parameters. It has been established that the ballistic regime is determined by molecular motors. The diffusive regime is of particular interest for inferring information about the cytoskeleton's role in transport.
The non-ballistic phases of intracellular transport are characterized experimentally using nanoparticles in Dictyostelium discoideum cells. Local MSD analysis yields the distributions for the effective diffusion coefficient and the local MSD exponent. These statistics can be reproduced by simulating a Brownian motion the increments of which are negatively correlated over short times. Very good agreement of the experimental and simulated statistics yields valuable information about the short-timescale subdiffusive behaviour and an underlying biophysical picture.