Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 5: Posters: Biopolymers and Biomaterials
BP 5.23: Poster
Montag, 22. März 2010, 17:15–20:00, Poster B1
Analysis of multivalent effects using pyridine coordination compounds in single molecule force spectroscopy (SMFS) — •Manuel Gensler1, Christian Eidamshaus2, Hans-Ulrich Reißig2, and Jürgen P. Rabe1 — 1Institut für Physik, Humboldt-Universität zu Berlin, Newtonstr. 15, 12489 Berlin — 2Institut für Chemie und Biochemie, Freie Universität Berlin, Takustr. 3, 14195 Berlin
Multivalent interactions are of great importance in supramolecular chemistry, nanotechnology or biochemistry [1]. They influence binding free energies and kinetics, which leads to strongly increased interaction strengths between partners of appropriate geometry. Thus it is important to obtain a deeper understanding of the basic factors influencing multivalent interactions.
SMFS provides a direct measurement of forces [2] and is therefore an ideal tool to study multivalency on the molecular level. We synthesized pyridine nanorods and coupled them to Au covered tips and surfaces, using thiol chemistry and PEG as spacer. Force-distance measurements at different loading rates were performed to estimate associated binding properties of mono- and multivalent coordination compounds with metal salts in aqueous solutions. Our model system can be extended to various solvents and geometries and therefore provide fundamental knowledge also for more complex biological and supramolecular systems.
[1] M. Mammen et al. Angew. Chem. Int. Ed. 37 (1998) 2754-2794.
[2] M.I. Gianotti et al. ChemPhysChem 8 (2007) 2290-2307.