Regensburg 2010 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 5: Posters: Biopolymers and Biomaterials

BP 5.25: Poster

Montag, 22. März 2010, 17:15–20:00, Poster B1

Microrheology: A system for the comparison of pancreatic carcinoma cells in an optical tweezers device and an electron microscope — •Tobias Paust¹, Anke Leitner¹, Ulla Nolte¹, Michael Beil², Paul Walther³, and Othmar Marti¹ — ¹Institute of Experimental Physics, Ulm University — ²Institute of Internal Medicine I, Ulm University Hospital — ³Central Electron Microscopy Unit, Ulm University

Nowadays the studies of pancreatic carcinoma cells are very important to get a insight into the behavior, structure and properties of these cells. Optical tweezers and microrheology methods allow to measure the mechanical properties of a selected cell. By tracing incorporated nanoparticles the trajectory of this particles and then the dynamic shear modulus of the cells or the a network can be determined. Electron microscopes can be used for resolving structures down to a nanometer level and clearly depict the pancreatic carcinoma cells to gather information about the arrangement of the network in the cell.
Combining both the microrheology and the pictures of the electron microscope allow to check whether the measurement at a specific cell was meaningful and the determined mechanical properties are appropriate input parameters for numeric simulations. For this purpose a method was developed for marking the position of the analyzed cell during the microrheology measurement and find again the same position in the electron microscope to examine the structure of the network and the positions of the traced beads.