Regensburg 2010 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 5: Posters: Biopolymers and Biomaterials
BP 5.34: Poster
Montag, 22. März 2010, 17:15–20:00, Poster B1
Self-assembled 3-dimensional DNA structures investigated with fluorescence microscopy — •Alexander Benkstein1, Zhao Wang1, Christoph Erben2, Iwan A. T. Schaap1, Andrew J. Turberfield2, and Christoph F. Schmidt1 — 1Drittes Physikalisches Institut, Fakultät für Physik, Georg-August Universität, 37077 Göttingen — 2Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK
Well established synthesis procedures and the "programmability" of DNA binding via base pairing makes DNA ideal for the design of nanostructures.
We here investigate the characteristics of self-assembled tetrahedra from DNA oligomers with dimensions smaller than 10 nm. We labeled tetrahedra with the intercalating dye YOYO-1 and imaged using total internal reflection fluorescence microscopy. We investigate the photobleaching of the dye bound to the DNA tetrahedron and estimate the number of dyes on the single structure.