Regensburg 2010 – scientific programme

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BP: Fachverband Biologische Physik

BP 5: Posters: Biopolymers and Biomaterials

BP 5.4: Poster

Monday, March 22, 2010, 17:15–20:00, Poster B1

Deep UV Raman spectroscopy on sensory rhodopsin — •Andreas Bröermann, Nils Priesnitz, Bernd Walkenfort, Johann Klare, Heinz-Jürgen Steinhoff, and Sebastian Schlücker — Fachbereich Physik, Universität Osnabrück, Barbarastr. 7, 49076 Osnabrück

The light-driven membrane pigment sensory rhodopsin II (SR II) consists of the protein opsin and the cofactor retinal. SR II is responsible for the negative phototaxis of halobacteria. Incident photons induce an isomerization of the retinal chromophore, which leads to a conformational change of opsin and, by means of a signalling cascade, finally controls the flagellar motor and thereby the swimming behavior of the cells.

The nano- to millisecond conformational dynamics of SR II can be probed by time-resolved UV resonance Raman scattering (UV RR) in a pump(VIS)-probe(deep UV) experiment with kHz repetition rate. The sample is excited by a frequency-doubled Nd:YAG laser (532 nm, 10 ns pulse length). After a variable time delay, the photo-excited SR II molecules are probed with deep UV laser radiation (195 nm, 10 ns pulse length). The deep UV Raman spectrum is recorded by a triple monochromator equipped with a gated image intensifier.