Regensburg 2010 – scientific programme
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BP: Fachverband Biologische Physik
BP 6: Posters: DNA and DNA Enzymes
BP 6.10: Poster
Monday, March 22, 2010, 17:15–20:00, Poster B1
Towards sub second imaging of DNA compaction by AFM — •Jan Knappe1, Sebastian Hanke1, Szabolcs Sörös2, Christoph F. Schmidt1, Wolfgang Fischle2, and Iwan A. T. Schaap1 — 1III. Physkalisches Institut, Georg-August-Universität, 37077 Göttingen — 2Max Planck Institute for Biophysical Chemistry, 37077 Göttingen
To compact and organize DNA the eukaryotic cell has developed several packaging steps. DNA is wrapped around histones to form nucleosomes, which in turn can be linked by other proteins to form larger aggregates. To study this system on a single molecule level we have set out to use atomic force microscopy (AFM) in liquid to image the binding and unbinding of DNA to the histone octamer. However to be able to study the dynamics of nucleosomal DNA on a biological relevant time scale, we need techniques that permit image acquisition in a second or faster. We will present our first results that combine imaging at low forces (~100 pN) with a high temporal resolution.