Regensburg 2010 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 6: Posters: DNA and DNA Enzymes

BP 6.4: Poster

Montag, 22. März 2010, 17:15–20:00, Poster B1

single-molecule force spectroscopy on phoB-DNA complexes — •Michael Birlo¹, Katrin Wollschläger², Rainer Eckel³, Norbert Sewald⁴, and Dario Anselmetti⁵ — ¹Department of Physics Bielefeld University 33615 Bielefeld (Germany) — ²Department of Chemistry Bielefeld University 33615 Bielefeld (Germany) — ³Department of Physics Bielefeld University 33615 Bielefeld (Germany) — ⁴Department of Chemistry Bielefeld University 33615 Bielefeld (Germany) — ⁵Department of Physics Bielefeld University 33615 Bielefeld (Germany)

Interactions between proteins and DNA are essential for the regulation of cellular processes in all living organisms. In this context, it is of special interest to investigate the sequence-specific molecular recognition between transcription factors and their cognate DNA sequences. As a model system, peptide and protein epitopes of the DNA-binding domain (DBD) of the transcription factor PhoB from Escherichia coli are analyzed with respect to DNA binding at the single-molecule level. Quantitative AFM-DFS analysis proves the specificity of the interaction and yields force-related properties and kinetic data, such as thermal dissociation rate constants. An alanine scan for strategic residues in both peptide and protein sequences is performed to reveal the contributions of single amino acid residues to the molecular-recognition process.