Regensburg 2010 – scientific programme
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 22: Biopolymers and Biomaterials (jointly with BP)
CPP 22.7: Talk
Wednesday, March 24, 2010, 16:00–16:15, H37
Biofunctionalization of Diamond Microelectrodes — •Andreas Adam Reitinger1, Naima Aurelia Hutter2, Simon Quartus Lud1, Gerhard Richter2, Rainer Jordan2, Martin Stutzmann1, and Jose Antonio Garrido1 — 1Walter Schottky Institut, TU München, Germany — 2WACKER-Chair of Macromolecular Chemistry, TU München, Germany
In this work we present two main routes for the biofunctionalization of nanocrystalline diamond films, aiming at the application of diamond microelectrodes as amperometric biosensors. We report on direct covalent grafting of biomolecules on nanocrystalline diamond films via diazonium monophenyls and biphenlys as well as other linker molecules, forming self-assembled monolayers on the diamond surface. Monolayers with different functional head groups have been characterized. Patterning of the available functional groups using electron beam-induced chemical lithography allows the selective preparation of well-localized docking sites for the immobilization of biomolecules. Furthermore, polymer brushes are expected to enable novel paths for designing more advanced biosensing schemes, incorporating multifunctional groups and a higher loading capacity for biomolecules. Here, we will focus on the preparation of polymer grafts by self-initiated photografting and photopolymerization. Further chemical modification of the grafted polymer brushes results in the introduction of additional functional molecules, paving the way for the incorporation of more complex molecular structures such as proteins. In a comparative study we investigate the advantages and disadvantages of both approaches.