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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 28: Poster: Biopolymers and Biomaterials

CPP 28.10: Poster

Mittwoch, 24. März 2010, 17:30–19:00, Poster C

Development of an Integrated System for High-Pressure NMR Spectroscopy on Proteins — •Werner Kremer, Markus Beck Erlach, Claudia E. Munte, T. Ernst, Rainer Hartl, M. Arnold, Dörte Rochelt, Dieter Niesner, and Hans Robert Kalbitzer — Institute of Biophysics and Physical Biochemistry, Regensburg

High hydrostatic pressure can induce multiple effects on proteins including denaturation, depolymerization, and changes of side chain protonation state. Pressure induced structural changes can be investigated with high pressure NMR spectroscopy, because different conformers in the energy-landscape of proteins are accessible via their different specific volume. Therefore static pressure in the range from 4-200 MPa has been applied to proteins and peptides. In addition the application of pressure jumps with a microprocessor controlled on-line pressure system has been performed in order to analyze possible structural intermediates which are not accessible by the utilization of static pressure. Quartz, sapphire or ceramic cells are used to handle the proteins in aqueous solutions during the experiment. The best results can be obtained with ceramic cells because they can withstand high pressures and can be easily handled. A completely new autoclave for these ceramic cells has been constructed, including an improved method for pressure transmission, an integrated safety jacket and a fast closing emergency valve.