Regensburg 2010 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 28: Poster: Biopolymers and Biomaterials
CPP 28.11: Poster
Mittwoch, 24. März 2010, 17:30–19:00, Poster C
A NMR Bioreactor Setup for 5 mm High-Resolution Probes at 800 MHz — •Paul Ramm1, 2, 3, Werner Kremer1, Ulrich Bogdahn2, Ludwig Aigner3, and Hans Robert Kalbitzer1 — 1Department of Biophysics and Biophysical Chemistry, University of Regensburg — 2Department of Neurology, University of Regensburg — 3Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg
NMR spectroscopy (MRS) of cells and tissues provides a quantitative insight into cellular composition and metabolism. A common approach is MRS of cell extracts, which can be used to distinguish e.g. different neural cell types [1]. MRS of living cells more closely resembles the in vivo situation, but is restricted due to unphysiological conditions in a NMR tube. Therefore, we are realizing a cell perfusion setup for a Bruker Avance 800 MHz spectrometer equipped with a 5 mm cryo probe. Immobilized cells can then be perfused with variably composed media saturated with a tunable gas mixture of N2, O2, and CO2. Highly-resolved 1H-NMR spectra (FWHM < 3 Hz) can be acquired with a temporal resolution < 5 min. Based on our former investigations on stem cell-specific biomarkers of neural progenitor cells (NPCs) [2], MRS of NPCs under controllable cell culture conditions will be the next step towards a metabolic profiling and biomarker screening of neurogenesis, leading to both a better understanding of stem cell metabolism and to a possible detection of neurogenesis in humans.
[1] Urenjak J et al., J Neurosci 13 (3), 1993
[2] Ramm P et al., Stem Cells 27 (2), 2009