Regensburg 2010 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Downloads | Hilfe

CPP: Fachverband Chemische Physik und Polymerphysik

CPP 3: Focus: Structure and Dynamics of Responsive Hydrogels I

CPP 3.5: Vortrag

Montag, 22. März 2010, 11:30–11:45, H37

New pNIPAM microgels for immobilization of proteins — •Kornelia Gawlitza, Marcel Richter, and Regine von Klitzing — TU Berlin, Stranski-Laboratorium, Strasse des 17. Juni 124, 10623 Berlin, Deutschland

Poly-N-isopropylacrylamide (pNIPAM) belongs to the group of the so called smart hydrogels and is one of the most studied water swellable microgel systems. Due to the reversibility of the volume phase transition at around 32^∘C, pNIPAM is qualified for applications like drug delivery and biocatalysts.^1,2

In our studies, we synthesized new pNIPAM microgels with suitable properties to reach an immobilization of proteins and hence a new biocatalyst. Therefore, we followed two different strategies: First, we tried to synthesize big hydrogels and second, we used the new comonomer Allylglycine (AG) as anchor points for the enzyme. The characterization of the synthesized microgels was done by Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM).

Furthermore, we used the received pNIPAM hydrogels for some immobilization experiments with BSA.^3,4 The following analysis of this experiments was done using the Bradford-assay in combination with fluorescence spectroscopy.

[1] K. Kratz, T. Hellweg, W. Eimer, Polymer (2001), 42, 6531 [2] R. Pelton, Adv. Colloid Interface Sci. (2000), 85, 1 [3] L. Bromberg, M. Temchenko, T. A. Hatton, Langmuir (2003), 19, 8675 [4] C. Yan, A. Elaissari, C. Pichot, J. Biomed. Nanotechnol. (2006), 2(3/4), 208-216