Regensburg 2010 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 38: Micro and Nanofluidics II
CPP 38.1: Vortrag
Donnerstag, 25. März 2010, 09:30–09:45, H39
Droplet and cell sorting in microfluidic channels by surface acoustic waves — Thomas Franke1,2, Lothar Schmid1, Susanne Braunmüller1, Achim Wixforth1, and •David Weitz2 — 1Universität Augsburg, EP1, Microfluidics Group, Augsburg — 2Harvard University, SEAS, Cambridge, USA
We direct the motion of droplets in microfluidic channels using a surface acoustic wave device. This method allows individual drops to be directed along separate microchannel paths at high volume flow rates, which is useful for droplet sorting. The same principle can be applied for biological cell sorting which operates in continuous flow at high sorting rates. The device is based on a surface acoustic wave cell-sorting scheme and combines many advantages of fluorescence activated cell sorting (FACS) and fluorescence activated droplet sorting (FADS) in microfluidic channels. It is fully integrated on a PDMS device, and allows fast electronic control of cell diversion. We direct cells by acoustic streaming excited by a surface acoustic wave which deflects the fluid independently of the contrast in material properties of deflected objects and the continuous phase; thus the device underlying principle works without additional enhancement of the sorting by prior labelling of the cells with responsive markers such as magnetic or polarizable beads. Single cells are sorted directly from bulk media at rates as fast as several kHz without prior encapsulation into liquid droplet compartments as in traditional FACS. We have successfully directed HaCaT cells, fibroplasts from mice and MV3 melanoma cells.