Regensburg 2010 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 46: Focus: Charge Effects in Soft and Biological Matter II (jointly with BP)
CPP 46.2: Vortrag
Donnerstag, 25. März 2010, 14:30–14:45, H37
Interaction of Proteins with Spherical Polyelectrolyte Brushes — •Katja Henzler1,2, Alexander Wittemann1, Björn Haupt1,2, Oleg Borisov3, and Matthias Ballauff2 — 1Universität Bayreuth, Physikalische Chemie I; Universitätstr. 30, 95440 Bayreuth, Germany — 2Helmholtz-Zentrum Berlin für Materialien und Energie; Hahn-Meitner-Platz 1; 14109 Berlin, Germany — 3Institut pluridisciplinaire de Recherche sur l’ Environement et les Materiaux, UMR 5254, CNRS/UPPA, 64053 Pau, France
Spherical polyelectrolyte brushes (SPB) are a novel class of carrier particles for the immobilization of proteins.[1] A high uptake of proteins can be achieved if the ionic strength is low, while both compounds carry an overall negative charge.[1] No adsorption takes place at higher ionic strength. The main driving force for the adsorption is the counterion release force. Counterions from the brush layer are released and the free energy of the system will be decreased. The thermodynamic of the described adsorption process can be investigated by isothermal titration calorimetry (ITC).[2] This method allows us to determine the adsorption isotherm together with the adsorption enthalpy and entropy. We demonstrated that the adsorption of β-lactoglobulin (BLG) onto the SPB is driven by a strong gain of entropy i.e. by the postulated uptake mechanism of the counterion release force. This is the first direct proof for the counterion release force.
Literature: [1] Wittemann, A.; Ballauff, M. Phys. Chem. Chem. Phys. 2006, 8, 5269. [2] Henzler, K.; Haupt, B.; Lauterbach, K.; Wittemann, A.; Borisov, O.; Ballauff, M. in preparation.