Regensburg 2010 – wissenschaftliches Programm
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O: Fachverband Oberflächenphysik
O 16: Organic, polymeric, biomolecular films - also with adsorbates
O 16.5: Vortrag
Montag, 22. März 2010, 16:00–16:15, H46
Fast and non-invasive optical study of protein films on surfaces — •Franz-Josef Schmitt1, Heinrich Südmeyer2, Kai Reineke1, Insa Kahlen1, Joachim Börner1, Max Schöngen1, Patrick Hätti1, Hans Joachim Eichler1, and Hans-Joachim Cappius2 — 1Berlin Institute of Technology, Berlin — 2Laser- und Medizintechnologie GmbH, Berlin
Thin films of proteins, bacteria and diverse biochemical compounds are always found on surfaces exposed to the environment. Optical methods deliver innovative tools for the study of protein films. Residue of proteins, which exhibit fluorescence when exited in the UV wavelength range, can easily be investigated optically. The results show that protein films are structured inhomogeneously. Ring shaped structures often found after surface drying can be explained by the influence of surface tension during the evaporation process of the protein solvents. We present a specially designed handheld measuring device that can analyse organic contamination levels below 1µg/cm2 within seconds. By using time-correlated methods the fluorescence detection of contamination level on surfaces is possible even if the fluorescence spectra of contamination and base material overlap.
Financial support by Federal Ministry of Education and Research (BMBF) is greatfully acknowledged.