Regensburg 2010 – wissenschaftliches Programm
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ST: Fachverband Strahlen- und Medizinphysik
ST 10: Basic and Applied Medical Physics (Poster Session)
ST 10.9: Poster
Mittwoch, 24. März 2010, 16:00–17:00, H41
Microfabricated flow cytometers for high throughput analysis of blood cells — •Denny Ragusch1, Marcin Frankowski1, Andreas Kummrow1, Nicole Bock1, Jörg Neukammer1, Andrej Tuchscheerer2, and Martin Schmidt2 — 1Physikalisch-Technische Bundesanstalt, Berlin — 2Technische Universität Berlin, Fachgebiet Mikro- und Feingeräte, Berlin
Microfluidic structures are of particular relevance as a part of simple and robust analytical systems for point-of-care in-vitro diagnostics. In flow cytometric analysis blood cells must be counted at relatively high throughput to assure sufficient statistics. Such systems require implementation of hydrodynamic focusing. Presented disposable measurement platforms allow for analysis and classification of human blood cells detecting scattered light, fluorescence and impedance changes. To overcome limitations of widely used photolithographic manufacturing methods, our approach utilizes mould inserts for hot embossing fabricated by ultra-precision milling [1]. The complex 3D polycarbonate structures featuring two-stage cascade hydrodynamic focusing enables measurements with count rates up to 5 kHz. Differentiation of CD14+ and CD3+/CD4+ cells clearly demonstrate the potential of using such microsystems. Optical excitation and detection was performed either by optical fibres or using external optics. Achieved pulse height distributions are comparable to those of conventional instruments. Measurements of fluorescence signals from calibration particles yield coefficients of variation of less than 2% for optimised settings.
[1] A. Kummrow et al, Lab Chip 9 (2009) 972-981