Dresden 2011 – scientific programme
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BP: Fachverband Biologische Physik
BP 10: Posters: Tissue Dynamics \& Developmental Processes
BP 10.5: Poster
Monday, March 14, 2011, 17:15–20:00, P3
In situ uv/vis spectroscopic imaging of retina cell degeneration — •Julia Hollmach1, Julia Schweizer1, Gerald Steiner1, Richard H. W. Funk2, Lilla Knels2, and Edmund Koch1 — 1Dresden University of Technology, Faculty of Medicine, Clinical Sensoring and Monitoring, Dresden, Germany — 2Dresden University of Technology, Faculty of Medicine, Anatomy, Dresden, Germany
In the western world retinal diseases like age-related macular degeneration have become an important cause of visual loss depending on increasing life expectancy and lifestyle habits. Since there is no sufficient treatment, early diagnosis and prevention are the only possibilities to preserve eyesight. The protein cytochrome c (cyt c) is a suitable marker for degeneration processes, because it is involved in the apoptosis pathway. In particular, the local distribution and oxidative state of cyt c are of clinical interest. Cyt c shows two overlapping absorption bands between 500 and 600 nm. Uv/vis spectroscopic imaging was used to characterize the oxidation state and the distribution of the protein in a layer of retina cells. The major challenge was the separation of molecular information from the scattering signal. Extended Multiplicative Scatter Correction in combination with Principal Component Analysis was performed to separate the signals in order to study spectral variances. After multivariate data analysis, cyt c could be identified. The imaging exhibits domains and 'hot spots' of cell degeneration processes. The results demonstrate that spectroscopic imaging in conjunction with sophisticated multivariate methods is a suitable tool to characterize degeneration processes under in situ conditions.