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BP: Fachverband Biologische Physik

BP 11: Posters: Single-Molecule Biophysics

BP 11.13: Poster

Montag, 14. März 2011, 17:15–20:00, P3

A theoretical description of the 3D orientation determination of dipoles near interfaces — •Richard Börner and Christian G. Hübner — Insitute of Physics, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany

One of the unique features of single molecule absorption/emission is their anisotropy due to the well-defined transition dipoles for both processes allowing the determination of the molecules 3d orientation. Therefore, several techniques have been proposed in order to determine the full three-dimensional orientation of dipole emitters on a single molecule level. One of these techniques proposed by Hohlbein and Hübner in 2005 combines emission distribution and polarization detection. The theoretical background of this approach assumes an optical homogenous surrounding. In order to overcome this limitation we want to indicate an extension of the theory for dipole emitters near optical interfaces e.g. glass/water. Moreover, we implement the dipole excitation probability. In conclusion, we present extensive simulations, which allow for the evaluation of the capabilities of this extended method.