Dresden 2011 – scientific programme
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BP: Fachverband Biologische Physik
BP 11: Posters: Single-Molecule Biophysics
BP 11.4: Poster
Monday, March 14, 2011, 17:15–20:00, P3
Analysis of multivalent effects using single molecule force spectroscopy (SMFS) on pyridine coordination compounds — •Manuel Gensler1, Artur Galstyan2, Ernst-Walter Knapp2, and Jürgen P. Rabe1 — 1Institut für Physik, Humboldt-Universität zu Berlin, Newtonstr. 15, 12489 Berlin — 2Institut für Chemie und Biochemie, Freie Universität Berlin, Fabeckstr. 36a, 14195 Berlin
Multivalent interactions are of great importance in chemistry, nanotechnology and biochemistry. They strongly increase binding free energies and association kinetics between partners of appropriate geometry [1]. Thus it is important to obtain a deeper understanding of the basic factors influencing multivalent interactions.
We used SFM based SMFS [2] to measure interaction forces between mono- and multivalent coordination compounds of pyridine nanorods with different metal salts such as Zn(NO3)2 and CuSO4in aqueous solutions. Force-distance measurements were performed over a broad range of loading rates to estimate associated binding properties according to the Bell-Evans model [3]. In combination with computational calculations of the bond dissociation under force we propose different rupture mechanisms of the divalent complexes with Copper and Zinc. Our model system can be extended to various geometries and therefore provides essential knowledge about geometrical factors influencing multivalency.
[1] M. Mammen et al. Angew. Chem. Int. Ed. 1998, 37, 20, 2754-2794. [2] M.I. Gianotti et al. ChemPhysChem 2007, 8, 2290-2307. [3] S. Guo et al. Biophys. J. 2008, 95, 3964-3976.