Dresden 2011 – scientific programme
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BP: Fachverband Biologische Physik
BP 11: Posters: Single-Molecule Biophysics
BP 11.5: Poster
Monday, March 14, 2011, 17:15–20:00, P3
High-Resolution Scanning Near-Field Optical Microscopy of Dye Labelled Single Tobacco Mosaic Viruses — •Alexander Harder1, Sven Degenhard2, Fabian Eber2, Fania Geiger3, Joachim Spatz2, Holger Jeske2, Christina Wege2, and Dario Anselmetti1 — 1Experimental Biophysics & Applied Nanoscience, Bielefeld University, Germany — 2Molecular Biology and Virology of Plant, Stuttgart University, Germany — 3Max Planck Institute for Metals Research, Stuttgart, Germany
Scanning near-field optical microscopy (SNOM) is a fluorescence microscopy technique achieving optical resolution of better than 20 nm by means of strongly confined non-propagating electromagnetic fields. We investigated dye-labelled single Tobacco mosaic viruses (TMV) with apertureless SNOM by using standard Si-AFM tips illuminating their apex with a focused laser beam. Our home-built SNOM device system additionally allows simultaneous atomic force microscopy (AFM) tapping topographic measurements [1]. In the future concurrent high structural and optical resolution will allow the investigation of virus orientation as well as site-specific immobilization that is prerequisite for possible bioengineering applications of TMV based channels.