Dresden 2011 – scientific programme
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BP: Fachverband Biologische Physik
BP 11: Posters: Single-Molecule Biophysics
BP 11.7: Poster
Monday, March 14, 2011, 17:15–20:00, P3
Single-Molecule Force Spectroscopy Binding Studies of DNA Recognition by Transcription Factor Epitopes — •Adeline Bieker1, Volker Walhorn1, Gesa Niemann2, Markus Ritzefeld2, Norbert Sewald2, and Dario Anselmetti1 — 1Experimental Biophysics and Applied Nanoscience, Universität Bielefeld, Deutschland — 2Organic and Bioorganic Chemistry, Universität Bielefeld, Deutschland
Interactions between proteins and DNA are essential for the regulation of cellular processes in all living organisms. In this context, it is of special interest to investigate and quantify the sequence-specific molecular recognition between peptidic transcription factors and their cognate DNA sequences [1].
We investigated protein epitopes and peptides originating from the DNA-binding domain (DBD) of the Escherichia coli transcription factor PhoB. By means of AFM-based Single Molecule Force Spectroscopy (SMFS) we investigated the specific binding forces and molecular elasticities to elucidate the DNA-protein complex stability. Based on the Bell-Evans-Model [2] we estimated the thermal dissociation rate constants ( koff) an the molecular interaction length ( xβ), that allowed a structure related interpretation of the physical binding mechanisms involved.