Dresden 2011 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 11: Posters: Single-Molecule Biophysics

BP 11.9: Poster

Montag, 14. März 2011, 17:15–20:00, P3

Stochastic reconstruction of interactions within protein complexes from single-molecule force spectroscopy — •Magnus Schwörer and Ulrich Gerland — Arnold Sommerfeld Center for Theoretical Physics, LMU München, Munich, Germany

Dynamic Force Spectroscopy is a well-established technique where a pulling force is applied with a certain rate of loading to probe the (un)folding of biomolecules or the interaction between two biomolecules. The technique typically permits to extract information such as the barrier height and distance to the transition state, and ideally even the entire free energy landscape along the reaction coordinate of this process. Here, we explore theoretically which information could be obtained when this technique is applied to macromolecular complexes. Specifically, we consider the sequential application of dynamic force spectroscopy to all pairs of constituents within such a complex, and test to which extent the interactions between the constituents can be reconstructed. Our analysis is based on a simple toy model.