Dresden 2011 – scientific programme

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BP: Fachverband Biologische Physik

BP 12: Posters: New Technologies

BP 12.14: Poster

Monday, March 14, 2011, 17:15–20:00, P3

Three-Focus-Fluorescence-Correlation-Spectroscopy (3fFCS) — •Lars Kreutzburg, Richard Börner, and Christian G. Hübner — Insitute of Physics, University of Lübeck, Ratzeburger Allee 160, 23562 Lübeck, Germany

Intracellular transport is achieved by the combination of diffusive and directed motion. FCS and its variants are well suited for the study of such processes. One of these variants presented by Dittrich and Schwille is the spatial-two-photon FCCS for one-dimensional flow measurements by shifting two detection volumes spatially in the precences of one large excitation volume. The later development of two-focus FCS (2fFCS) by Dertinger et al. allows for the determination of exact diffusion coefficients by splitting the optical pathways of two orthogonally polarized excitation beams via a DIC prism, which leads to two spatial shifted overlapping confocal volumes. Following these approaches, we propose a new variant, called three-focus FCS, which enables for the determination of the direction of directed motion of molecules by shifting three or more detection volumes relatively to one excitation spot. We will present a theoretical description of 3fFCS. Moreover, extensive simulations allow for the evaluation of the capabilities of this method. The simulations are compared with the first experimental results.