Dresden 2011 – scientific programme

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BP: Fachverband Biologische Physik

BP 12: Posters: New Technologies

BP 12.16: Poster

Monday, March 14, 2011, 17:15–20:00, P3

(contribution withdrawn) Upgrading a Commercial Confocal Microscope to CW-STED Super-Resolution — •Til Driehorst, Florian Rehfeldt, and Christoph F. Schmidt — Drittes Physikalisches Institut, Georg-August-Universität Göttingen, Germany

Fluorescence microscopy is one of the most commonly used imaging techniques in the life sciences, particularly when investigating living organisms at the sub-cellular level. A major drawback is the diffraction-limited resolution. This limit has been overcome by several new methods such as stimulated emission depletion (STED) microscopy. Here we describe the upgrade of a Leica TCS SP5 X confocal microscope to super-resolution by implementing a custom-built STED system. Fluorophore excitation is done with a pulsed white light laser (WLL) source, while a 592 nm continuous wave (CW) laser is used for STED. The combination of a WLL source and 592 nm STED laser is well-suited for commonly used fluorescent markers such as the FITC and the yellow fluorescent protein (YFP) family.