Dresden 2011 – scientific programme

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BP: Fachverband Biologische Physik

BP 12: Posters: New Technologies

BP 12.18: Poster

Monday, March 14, 2011, 17:15–20:00, P3

Analytic solution for image analysis in localization microscopy with full accuracy — •Frederik Grüll, Manfred Kirchgessner, and Udo Kebschull — Kirchhoff Institute für Physics, Heidelberg University, Germany

In localization microscopy the resolution limit is improved by calculating the centroid of the image of each fluorescent point-like object. Current solutions obtain the center by fitting a Normal Distribution and optimize the maximum likelihood or least squares iteratively. Faster analytical approaches exist, but come with a reduced precision in noisy environments. We propose an algorithm that is based on maximum likelihood, but solves the problem analytically. Results show that we maintain full accuracy also for noisy images with a speedup of more than 100 compared to numerical fits, and provide an accurate error estimation for each localization. As a consequence image analysis for localization microscopy becomes real-time capable on standard computer hardware.