DPG Phi
Verhandlungen
Verhandlungen
DPG

Dresden 2011 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

BP: Fachverband Biologische Physik

BP 13: Posters: Biological Membranes

BP 13.11: Poster

Montag, 14. März 2011, 17:15–20:00, P3

Crystallinity of purple membranes comprising the chloride-pumping bacteriorhodopsin variant D85T — •Daniel Rhinow1, Ivan Chizhic2, Roelf-Peter Baumann2, Frank Noll2, and Norbert Hampp21Max-Planck-Institut für Biophysik, Max-von-Laue-Str. 3, 60438 Frankfurt — 2Philipps-Universität Marburg, Fachbereich Chemie, Hans-Meerwein-Str., 35032 Marburg

Purple membranes (PM) from Halobacterium salinarum comprise bacteriorhodopsin (BR) and lipids only and form a 2-D crystalline lattice in the cell membrane. In PMs comprising the chloride-pumping BR-variant D85T we have observed a tuneable tendency to form crystalline domains, which depends on pH-value and chloride ion concentration. We have combined small angle X-ray scattering, atomic force microscopy and freeze-fracture electron microscopy to analyze structural transitions within PM-D85T statistically as well as on the single membrane level. PM-D85T is a model system to study membrane protein association upon substrate binding in a native environment.

100% | Mobil-Ansicht | English Version | Kontakt/Impressum/Datenschutz
DPG-Physik > DPG-Verhandlungen > 2011 > Dresden