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BP: Fachverband Biologische Physik

BP 16: Biological Membranes I

BP 16.1: Hauptvortrag

Dienstag, 15. März 2011, 10:15–10:45, ZEU 260

The dynamic organization in the membrane of a G-protein-coupled receptor is related to its functional state — •Laurence Salomé — IPBS, Toulouse, France

The analysis of membrane diffusion is the most promising approach to investigate the compartmentalization of G-protein-coupled receptors, particularly as relevant to receptor signalling processes. We developed two complementary techniques: the fluorescence recovery after photobleaching (FRAP) performed at variable spot radius and the single particle tracking (SPT). We report the results of our study of a G-protein-coupled receptor involved in pain treatment, the human mu-opioid receptor (hMOR), using these techniques. We will survey the effects of the presence of distinct agonist ligands, antagonist ligand or the activation of other receptors on the diffusional behaviour of the receptor. Our observations suggest that the functional state of a receptor is correlated to its dynamic organisation in the plasma membrane.