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BP: Fachverband Biologische Physik

BP 20: Neurobiophysics

BP 20.2: Vortrag

Mittwoch, 16. März 2011, 10:45–11:00, ZEU 250

Optically Clamping Neurons in vitro — •Kai Bröking^1,3,5, Ahmed ElHady^1,2,4,5, Ragnar Fleischmann¹, Theo Geisel^1,3,4,5, Walter Stühmer^2,4,5, and Fred Wolf^1,3,4,5 — ¹Max-Planck-Inst. für Dynamik und Selbstorganisation, Göttingen, Germany — ²Max-Planck-Inst. für experimentelle Medizin, Göttingen — ³Fakultät für Physik, Georg-August-Universität Göttingen — ⁴Bernstein Center for Computational Neuroscience, Göttingen — ⁵Bernstein Focus for Neurotechnology, Göttingen

Transfecting neurons to express the light-gated ion channel Channelrhodopsin2 (ChR2) makes it possible to influence their activity noninvasively, by means of photostimulation [1]. We have implemented a feedback control system using optical stimulation at λ ≈ 480 nm which can be used to regulate the firing rate of neural networks cultured on multielectrode arrays. Our system allows closed loop feedback on timescales comparable to those of synaptic response (1−5 ms). We present an experimental setup for adjusting the average firing rate of neurons by means of feedback controlled photostimulation, thus devising a way of optically clamping an ensemble of cells. [1] Boyden, E., et al., Nat Neurosci 8, 1263-1268, doi:10.1038/nn1525