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BP: Fachverband Biologische Physik

BP 21: Biopolymers and Biomaterials II (with CPP)

BP 21.7: Vortrag

Mittwoch, 16. März 2011, 12:15–12:30, ZEU 260

Functionalized lipid bilayers for rapid cell attachment — •Samira Hertrich, Martin Huth, and Bert Nickel — Ludwig-Maximilians-Universität, Department für Physik and CeNS, Geschwister-Scholl-Platz 1, 80539 München

The behaviour of cells in contact to interfaces varies significantly depending on the surface properties. Bioadhesive coatings can act as an interlayer between cells and anorganic interfaces tuning the interaction of cells with the surface. Here, a multilayer system consisting of a supported lipid bilayer and two protein layers is used to produce a surface favoring neural stem cell attachment. Biotin and streptavidin act as a layer of linkers in between the lipid bilayer and the cell adhesive polypeptide AK-cyclo[RGDfC].

The trilayer system was characterised by x-ray reflectometry (D4, HASYLAB) and neutron reflectometry (N-REX, FRM2) measurements, which allowed for the determination of the layers thicknesses and the hydration of both lipids and proteins. Cell attachment to the coated surface was verified via fluorescence microscopy [1]. Microscopy confirms rapid attachment of stem cells while reflectometry indicated a dense on edge configuration of the AK-cyclo[RGDfC] thus maximizing the number of exposed RGD groups. Experiments have been performed in collaboration with E. Madarasz and G. Menzo from the Hungarian Academy of Science (HAS).

[1] Huth, M, et al., Materials 2010, 3, 4994-5006.