Dresden 2011 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 24: Physics of Cells II
BP 24.7: Vortrag
Donnerstag, 17. März 2011, 12:00–12:15, ZEU 250
Anomalous diffusion of intracellular lipid granules — •Christine Selhuber-Unkel1,2, Pernille Yde2, Jae-Hyung Jeon3, Vincent Tejedor3, Kirstine Berg-Sorensen4, Ralf Metzler3, and Lene B. Oddershede2 — 1University of Kiel, Institute for Materials Science — 2University of Copenhagen, Niels-Bohr-Institute — 3Technical University Munich, Physik-Department — 4Technical University of Denmark, Department of Physics, Kgs. Lyngby
The intracellular motion of cellular compartments plays an essential role for directed and undirected intracellular transport processes. We used live cell imaging and optical tweezers to track single endogenous, intracellular particles with high temporal and spatial resolution in order to investigate the diffusion properties of the granules in the different phases of the cell cycle. We found that the majority of the lipid granules underwent subdiffusive motion during all stages of the cell cycle. Interestingly, our results indicate that the cytoplasm is more elastic during interphase than during cell division and that its elasticity is relatively constant during the stages of cell division. In interphase, a comparison of our data with complementary analytical results has shown evidence for anomalous diffusion and ageing. We demonstrate that in the millisecond regime the granules follow subdiffusive motion according to the laws of continuous time random walk theory. At longer times granule motion is consistent with fractional Brownian motion.