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BP: Fachverband Biologische Physik

BP 29: Posters: Biopolymers \& Biomaterials

BP 29.22: Poster

Donnerstag, 17. März 2011, 17:15–20:00, P3

Towards Non-Invasive Cell Sorting and Specific Insights into Differential Hsp70 Expression in Colon Carcinoma Sublines — •Patrice Donfack¹, Gabriele Multhoff², and Arnulf Materny¹ — ¹Center of Functional Materials and Nanomolecular Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany — ²Dpt. Radiation Oncology, Klinikum rechts der Isar, TU München, Germany

Raman scattering provides noninvasive discrimination and biomolecular insights into Hsp70 associated with human colon carcinoma sublines CX- and CX+ in-vivo. CX-/CX+ are different in Hsp70 membrane expression exhibiting biologically relevant chaperon functions with immunostimulatory effects. We have phenotypically characterized them by immunofluorescence and Raman spectroscopy, combined with robust clustering and multivariate analyses. Although, CX- and CX+ show similar Raman spectra due to their strong resemblance, their protein-dominated Raman spectra reveal changes in protein and amino acids. Also significant and specific changes in DNA/RNA nucleotides involve pyrimidine rings Raman hypochromic effects. Discriminating CX- from CX+ is ultimately achieved with one or two principal components, paving the way for label-free cell-sorting of the sublines. Changes in proteins point to tumor-specific interactions of regulatory proteins and changes in nucleobases indicate DNA-RNA/protein binding interactions. We suspect transcription deregulations as participating precursor onsets of different transport mechanisms leading to Hsp70 differential expression and associated CX-/CX+ phenotypic variation.