Dresden 2011 – scientific programme

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BP: Fachverband Biologische Physik

BP 30: Posters: Physics of Cells

BP 30.18: Poster

Thursday, March 17, 2011, 17:15–20:00, P3

Correlative Microscopy: On the position of extracted pancreatic carcinoma cells — •Tobias Paust¹, Thomas Föhlich¹, Samuel Vollmer¹, Tobias Pusch¹, Paul Walther², Michael Beil³, and Othmar Marti¹ — ¹Institute of Experimental Physics, Ulm University — ²Central Electron Microscopy Unit, Ulm University — ³Department of Internal Medicine I, Ulm University

Microrheology measurements of particles which are embedded in the cytoskeleton of extracted carcinoma cells show the mechanical properties of the network. The network stiffness then can be calculated dependent on the position of the bead.

To ensure that a measurement of a cells shows up correct values for storage and loss modulus the same cell should be checked in the Electron Microscope. Therefore we developed a sample chamber which makes it possible to find the position of the microrheological measurement in the Electron Microscope. So only particles in a proper position can be used for calculations.

On this poster we want to show how to find cells and also take a look on the statistics on the amount of lost cells during the preparation process for the Electron Microscope.