Dresden 2011 – scientific programme
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BP: Fachverband Biologische Physik
BP 30: Posters: Physics of Cells
BP 30.21: Poster
Thursday, March 17, 2011, 17:15–20:00, P3
Regulation of Dynamic Cell Response with Laterally Confined Domains Embedded in Supported Membranes — •Thomas Kaindl1, Stefan Kaufmann1, Oleg Konovalov2, Ana Martin-Villalba3, and Motomu Tanaka1 — 1PCI, Universität Heidelberg, Germany — 2ESRF, Grenoble, France — 3DKFZ, Heidelberg, Germany
Highly uniform and strongly correlated domains of synthetic, fluorinated lipids were incorporated into solid supported lipid membranes to act as the confined, multivalent sites to regulate cell surface interactions. It was demonstrated that fluorinated lipids form mono-dispersive domains whose domain size and inter-domain correlation can precisely be controlled by the length of fluorocarbon chains. The fluorinated lipid domains were modified with carbohydrates or an apoptosis-inducing protein ligand (CD95L) which could successfully activate the specific cell response of macrophages and cancer cells. The dynamic spreading of murine macrophage and apoptosis of pancreatic cancer cells were analyzed by a combination of confocal microscopy and reflection interference contrast microscopy (RICM). The lateral confinement of ligand molecules revealed a significant effect on the adhesion behavior of cells.