Dresden 2011 – scientific programme
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BP: Fachverband Biologische Physik
BP 30: Posters: Physics of Cells
BP 30.8: Poster
Thursday, March 17, 2011, 17:15–20:00, P3
Nanosized vesicle transport in quasi 1D prepatterned Human Umbilical Vein Endothelial Cells (HUVEC): projecting 2D trajectories into 1D — •Marion Vollmer1, Matthias Himmelstoß1, Stefan Zahler2, and Doris Heinrich1 — 1Faculty of Physics and Center for NanoSciences (CeNS), Ludwig-Maximilians-University, Geschwister-Scholl-Platz 1, 80539 Munich, Germany. — 2Center for Drug Research, Pharmaceutical Biology, Ludwig-Maximilians-University, 81377 Munich, Germany.
Intracellular transport is a regulated process to orchestrate the localisation of vesicles to subcellular compartments. Vesicles attach to microtubules (MT) via motor proteins and are transported to their destination. This directed transport is intercepted by the dissociation of the vesicles from the MT leading to diffusive motion. To distinguish between directed and diffusive motion in the cell, our group developed the TRAnSpORT algorithm [1], based on the analysis of the mean square displacement (MSD). To calculate the vesicular run lengths more precisely we projected 2D trajectories into 1D, thereby reconstructing the MT virtually. This approach was tested in HUVEC that were grown on prepatterned surfaces, generating elongated, quasi-1D cell shapes and which were treated with low doses of the antimicrotubule drug vinblastine. Compared to untreated cells, vesicles in treated HUVEC showed a reduced run length and velocity, indicating the reduction of directed transport processes. This suggests, that the 1D projection is a precise tool to analyse curvilinear trajectories with changing directions of motion. [1] Arcizet et al., PRL, 101(24):248103, 2008.