Dresden 2011 – scientific programme

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BP: Fachverband Biologische Physik

BP 34: New Technologies

BP 34.3: Talk

Friday, March 18, 2011, 11:00–11:15, ZEU 260

Distance measurements in the nanometer range by in-cell EPR — •Malte Drescher — Emmy-Noether-Gruppe Physikalische Chemie, Universität Konstanz, 78457 Konstanz

In the past years, Electron paramagnetic resonance (EPR)spectroscopy has witnessed tremendous methodological and instrumental developments. These new methods have strong impact on biostructural research. Diamagnetic material such as most biomacromolecules (DNA, proteins, etc.)can be investigated by site-directed spin-labeling. Of particular interest are Double Electron Electron Resonance (DEER) techniques giving access to inter- and intramolecular distance distributions in the nanometer range. Dynamics can be monitored on a scale from pico- to microseconds. The utilization of pulsed EPR methods is especially useful for addressing structural features in complex systems.

In particular, our current effort to go beyond in-vitro approaches and to in-cell EPR will be introduced. Distance measurements by in-cell EPR using a spin-labeled DNA model system will be demonstrated.

[1] S. Domingo Köhler, A. Weber, S. P. Howard, W. Welte, and M. Drescher, Protein Science 19 (2010) 625-630 [2] M. Robotta, P. Braun, B. van Rooijen, V. Subramaniam, M. Huber, and M. Drescher, ChemPhysChem (2010) accepted [3] V. Singh, M. Azarkh, T. Exner, J. Hartig, M. Drescher Angewandte Chemie Int. Ed. 48 (2009) 9728-9730