Dresden 2011 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 34: New Technologies
BP 34.4: Vortrag
Freitag, 18. März 2011, 11:15–11:30, ZEU 260
Optical manipulation of neuronal networks bursting dynamics — •Ghazaleh Afshar1,3, Ahmed El Hady1,2,3, Theo Geisel1,3,4,5, Walter Stuehmer2,3,4, and Fred Wolf1,3,4,5 — 1Max Planck Institute for Dynamics and Self organization, Göttingen, Germany — 2Max Planck Institute of Experimental Medicine, Göttingen, Germany — 3Bernstein Focus for Neurotechnology, Göttingen, Germany — 4Bernstein Center for Computational Neuroscience, Göttingen, Germany — 5Faculty of Physics, Georg August University, Göttingen, Germany
Cultures of channelrhodopsin-2 transfected hippocampal neurons allow simultaneous optical stimulation and electrical recording from neuronal networks. As has been previously reported, after maturation, these networks develop an electrical activity that is characterized by synchronized bursts. In this work, we study the influence of whole field blue light illumination on burst dynamics of these cultures. During stimulation the mean firing rate is significantly different than before and after stimulation. Moreover, the mean spike rate during burst is significantly higher during stimulation. After turning off the stimulus, a silent period follows and then the network gradually switches into an ongoing state of bursting activity with even higher mean spike rate during stimulation. In fact, the mean duration of burst decreases during and after stimulation compared to non-perturbed spontaneous activity. We conclude that light stimulation can be used to persistently influence bursting dynamics in biological neuronal networks.