Dresden 2011 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 34: New Technologies

BP 34.7: Vortrag

Freitag, 18. März 2011, 12:15–12:30, ZEU 260

Enhancing the penetration depth in biological matter using Microscopy with Self-Reconstructing Beams — •Cristian Gohn-Kreuz and Alexander Rohrbach — University of Freiburg, Laboratory for Bio- and Nano-Photonics, 79110 Freiburg, Germany

Microscopy with Self-Reconstructing Beams (MISERB) is an imaging technique derived from the concept of light-sheet based microscopy. In this technique optical sectioning, i.e. the avoidance of out-of-focus light, is achieved by creating a thin sheet of light within a fluorescently labeled sample, while detecting the emitted light in a direction perpendicular to the illumination axis. In contrast to regular light sheet based microscopy, where the light sheet is either created by a cylindrical lens or a laterally scanned Gaussian beam, the light sheet in MISERB is created by a laterally scanned Bessel beam [1]. This beam belongs to the class of self-reconstructing beams. It recuperates its beam profile a short distance behind a scattering object. Due to this fact Bessel beams can provide a higher penetration depth than Gaussian beams in dense biological media like e.g. human skin. The penetration depth and image quality deep inside an object however is still limited by scattering artifacts. These artifacts result from the fact that the original beam profile gets distorted while propagating through thick scattering media. In this work we will investigate the controlled reduction of scattering artifacts by individually matching the illumination beam to the sample under consideration, thus enhancing the penetration depth in thick scattering media. [1] F. O. Fahrbach, P. Simon, and A. Rohrbach, Nature Photonics 4, 780-785 (2010)