Dresden 2011 – scientific programme
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BP: Fachverband Biologische Physik
BP 8: Posters: Protein Structure \& Dynamics
BP 8.1: Poster
Monday, March 14, 2011, 17:15–20:00, P3
Conformational Adsorption Reaction of BSA on the Surfaces of Nanosilica and Nanodiamond — •Victor Wei-Keh Wu — Department of Chemical and Materials Engineering, National Kaohsiung University of Applied Sciences(KUAS), 80782 Kaohsiung City, Taiwan — Victor Basic Research Laboratory e. V.(VBR) Gadderbaumer-Str. 22, D-33602 Bielefeld, Germany
From the fluorescences (excitation at 280 nm) of BSA of 0-10000 nM in 7 mM PPBS at pH=4.70 before and after adsorption reactions on the surfaces of nanosilica(NS) and nanodiamond(ND) of diameter 100 nm as suspension solutions(50µg/20µL), the adsorption thresholds, reaction constants as well as coverages have been obtained. Adsorption thresholds were located at 150 nM for both systems. The adsorbed BSA were 420 mg for two partices in g; 28.0 and 7.64 mg, on unit surface in m2 of NS and ND, respectively. Adsorption constants 1.2x108 and 3.7x107 (nM)−1 for systems BSA+NS and BSA+ND, respectively, have been obtained. Comparing with the respective constants 1.2x107 and 6.5x107 for systems lysozyme+NS and lysozyme+ND, the biomolecular conformations as well as behaviors are different. The spatial fitting between depression or hole on the nanosurface as carrier, and dimension of the protein with significant activity should also be considered, besides the charge-charge interactions between the surface and protein, and among the proteins. Financial aids by groups 510 and NB11 of IAMS, Taipei, and VBR, Bielefeld; support with Fluorescence Spectrophotometer F-4500 FL, Hitachi, Japan, by KUAS are acknowledged. Ref. V. W.-K. Wu and F. Kure, Chin. J. Chem. 28(2010).