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BP: Fachverband Biologische Physik

BP 9: Posters: DNA \& DNA Enzymes

BP 9.4: Poster

Montag, 14. März 2011, 17:15–20:00, P3

Information transfer and readout in complex DNA mixtures — •Harish Bokkasam and Albrecht Ott — Institute for Biological Experimental Physics, University of Saarland, Saarbruecken, Germany

Project: Development of an enzyme based method for the copy of oligos with predetermined length form biological template, given knowledge of the therein contained oligonucleotide sequence.

In this project, we modify the conventional PCR technique by using single primers to generate linearly amplified copies of single stranded oligos. This way the timescale of temperature cycle, which determines the length of the transcribed sequence is easier to control.

Results & Discussion: We find that single stranded DNA oligos of length b/w 40bp-200bp can be generated using this method. In order to determine the accuracy of the method the ssDNA is hybridised on a DNA coated surface with complementary sequence. We have shown that the time course of the hybridisation is almost identical to an error free sequence. This suggests the fidelity of the transcription.

Conclusion: Our method has given very promising results so far. Currently we are performing experiments along two lines: 1) Validate our technique by transcribing multiple single DNA sequences from a complex mixture. 2) Testing a different enzymes and polymerases for isothermal amplification and controlled extension of primers into short oligos. This will further improve the yield and also narrow the length distribution of the obtained products.