Dresden 2011 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 9: Posters: DNA \& DNA Enzymes

BP 9.6: Poster

Montag, 14. März 2011, 17:15–20:00, P3

Modelling the recruitment of DNA repair enzymes — •Gregor Weiss, Daniel Löb, and Barbara Drossel — Institut für Festkörperphysik, TU Darmstadt

We investigate the recruitment dynamics of repair enzymes during Base Excision Repair (BER) of DNA damage. Our focus lies on the possible competition of the enzyme loading platforms XRCC1 and PCNA in short patch BER. We also include Poly(ADP-ribose)polymerase-1 (PARP-1) in the model, which is indispensable for XRCC1 association with the DNA lesion.

We construct different possible models for the recruitment to DNA damage and dissociation of these three proteins, and perform numerical simulations of the models. In order to decide which models are more realistic, the simulation data are compared to empirical data obtained in living cells obtained using GFP-tagged proteins. Furthermore, these models are used to simulate the effect of protein inhibition, and to obtain more generally a relation between various model ingredients and signatures of the protein recruitment data curves.