Dresden 2011 – scientific programme
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 28: Biopolymers and Biomaterials II (jointly with BP)
CPP 28.8: Talk
Wednesday, March 16, 2011, 12:30–12:45, ZEU 260
Two-component Polymer Scaffolds for Controlled Three-dimensional Cell Culture — •Benjamin Richter1,2, Franziska Klein1, Thomas Striebel1, Clemens Franz1, Georg von Freymann3, Martin Wegener2, and Martin Bastmeyer1 — 1Zoologisches Institut, Karlsruher Institut für Technologie, 76131 Karlsruhe — 2Angewandte Physik, Karlsruher Institut für Technologie, 76131 Karlsruhe — 3AG Optische Technologien und Photonik, Technische Universität Kaiserslautern, 67663 Kaiserslautern
Fibrous collagen or matrigel matrices are commonly used to study three-dimensional (3D) cell behaviour, but these matrices have a random pore size and are structurally and chemically ill defined. We and others have recently shown that direct laser writing (DLW) is a versatile technique to fabricate tailored 3D cell-culture scaffolds in the micrometer to nanometer range. By using an adequate photoresist, elastic 3D scaffolds for cell-force measurements have also been realized. These DLW scaffolds have been homogeneously coated with ECM molecules. Ideally, they should rather have an adjustable distribution of cell-substrate contact sites to manipulate cell adhesion and cell shape in all three dimensions. By sequential DLW of two different photoresists, composite-polymer scaffolds with distinct protein-binding properties are fabricated and selectively bio-functionalised thereafter. Cells cultured in these scaffolds selectively form cell-adhesion sites with the functionalised parts, allowing for controlling cell adhesion and cell shape in 3D - forming the basis for future designer tissue-culture scaffolds.