Dresden 2011 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 35: Poster: Micro- and Nanofluidics

CPP 35.17: Poster

Wednesday, March 16, 2011, 17:00–19:00, P2

Single cell protein analysis via two-color UV/VIS laser induced fluorescence detection in microfluidic devices — •Lukas Galla, Dominik Greif, Dario Anselmetti, and Jan Regtmeier — Experimental Biophysics & Applied Nanoscience, Bielefeld University, Germany

In order to investigate and quantify the individual and heterogeneous cellular response, single cell analysis promises individual expression studies, which are not limited by ensemble averaging effects [1].

Upon extending our previous work on UV-LIF studies of single cells and protein separation at ultra-low analyte concentrations [2], we present a novel detection setup that allows the simultaneous recording of UV and VIS fluorescence signals at a ms timescale in microfluidic devices.

As first results we demonstrate protein fingerprints of Spodoptera frugiperda insect cells (Sf9) and chinese hamster ovary cells (CHO) in UV and VIS spectral range, demonstrating the feasibility of the parallel detection. [1] J. El-Ali et al., Nature 442, 403, (2006),[2] D. Greif et al., J. of Chromatography A 1206 (1), 83, (2008).