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Dresden 2011 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 44: Micro- and Nanofluidics I

CPP 44.3: Talk

Thursday, March 17, 2011, 14:30–14:45, ZEU 160

A microfluidic ratchet device for the controlled and tuneable separation of microparticles — •Lukas Bogunovic1, Ralf Eichhorn2, Jan Regtmeier1, Dario Anselmetti1, and Peter Reimann11Bielefeld University, Bielefeld, Germany — 2NORDITA, Stockholm, Sweden

Standard biotechnological separation techniques - such as gel electrophoresis - are often restricted to a separation criterion that is unchangeably implemented during fabrication. Therefore a new microfluidic device, based on a dielectrophoretic (DEP) ratchet design, has been designed and characterized [ 1 ]. It allows the dynamic and selective adaption of the separation criterion and direction of migration during operation. As a proof-of-concept, we demonstrate the separation of one arbitrary micro bead species out of three different kinds with freely selectable direction of migration for each species. The device consists of a straight microfluidic channel structured with an array of triangles. Here, DEP traps are created by an alternating voltage UAC while a constant voltage UDC drives negatively charged particles with diameters d1=1.1µm, d2=1.9µm and d3=2.9µm through the channel by electrophoresis (EP). Depending on the relative strengths of EP and DEP forces, a selection of particle types that will be trapped and those, which will still be transported can be archived. Combining these system states to a tailored driving protocol leads to the desired separation task.
[ 1 ] L. Bogunovic et al. "A tuneable microfluidic ratchet for particle sorting", submitted 2010

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