Dresden 2011 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
DS: Fachverband Dünne Schichten
DS 65: Organic Thin Films IV
DS 65.3: Vortrag
Freitag, 18. März 2011, 16:30–16:45, GER 38
Online monitoring of the adsorption and release of proteins on thin organic functional surfaces using infrared spectroscopic ellipsometry — •Dennis Aulich1, Eva Bittrich2, Klaus-Jochen Eichhorn2, Petra Uhlmann2, Manfred Stamm2, Norbert Esser1, and Karsten Hinrichs1 — 1Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V., Albert-Einstein-Str.9, 12489 Berlin, Germany — 2Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Str. 6, 01069 Dresden, Germany
Infrared spectroscopic ellipsometry (IRSE) is a well established method for optical and structural analysis of ultra-thin organic films. However, in-situ measurements in the infrared range are limited in aqueous environments due to the low penetration depth of infrared radiation in water. An in-situ cell which was developed at ISAS in Berlin allows IRSE measurements of ultra-thin organic films in aqueous environments by probing the interface region through the back side of a silicon wedge. The adsorption and release of proteins such as human serum albumin (HSA) on functional surfaces was studied using in-situ IRSE. A polyelectrolyte brush prepared from poly(acrylic acid) was used for the controlled adsorption and release of the protein. The thickness of this functional surface was about 5 nm. Adsorption and release was controlled by adjusting the pH of an aqueous solution and monitored by measuring the amid I and II bands of the protein.