Dresden 2011 – wissenschaftliches Programm

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MA: Fachverband Magnetismus

MA 8: Magnetic Particles/ Clusters I

MA 8.3: Vortrag

Montag, 14. März 2011, 15:15–15:30, HSZ 103

GMR-based real-time cell endocytosis monitoring of magnetic particles — •Astrit Shoshi, Joerg Schotter, Peter Ertl, Philipp Schroeder, Moritz Eggeling, Marcus Milnera, Verena Charwat, Florian Bellutti, Michaela Purtscher, Alexandra Keller, and Hubert Brueckl — AIT Austrian Institute of Technology GmbH; Nano Systems, Donau-City-Strasse 1, 1220 Vienna, Austria

We present a Magnetic-Lab-on-a-Chip-System (MAGLab-System) which provides a platform for on-chip cell analysis. Cell analysis is carried out by the interplay between giant magnetoresistive sensors (GMR-Sensors), superparamagnetic particles (beads) and magnetic fields in a microfluidic environment. The GMR-sensors are embedded in a silicon chip and provide electronic signals proportional to their bead surface coverage. GMR-based real-time monitoring of cell endocytosis is realized by first immobilizing magnetic beads onto the GMR-sensor surface. Second, human fibroblast cells (NHDF) are grown over the entire sensor surface to a confluent monolayer. After bead recognition, the cells start to engulf and internalize them into phagosomes. During this uptake process, the distance between the beads and the sensor increases, leading to lower stray field strengths and smaller sensor signals. Real-time monitoring of the entire dynamic uptake process is realized by reading out the sensors at appropriate time-intervals. By following a similar approach, on-chip cell migration, adhesion, detachment and magnetic manipulation have also been demonstrated successfully.