Dresden 2011 – scientific programme

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MI: Fachverband Mikrosonden

MI 5: X-Ray Spectrometry and Tomography

MI 5.8: Talk

Tuesday, March 15, 2011, 12:15–12:30, BEY 81

X-ray nano-tomography and nano-spectroscopy with the HZB X-ray microscope — •Peter Guttmann, Stefan Rehbein, Stephan Werner, and Gerd Schneider — Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Wilhelm-Conrad-Röntgen-Campus, Albert-Einstein-Str. 15, 12489 Berlin, Germany

The HZB X-ray microscope at the undulator beamline U41 at BESSY II is a unique setup allowing cryo-tomography of biological samples to reveal 3D ultra-cellular structures without staining or sectioning of the samples [1]. Flat sample holders can be used which allows the investigation of e.g. adherent mammalian cells. The resolution for tomographic data sets of real biological samples was determined to 36 nm (Rayleigh criterion). For 2D images 11 nm lines and spaces can be resolved by using a higher order of the zone plate objective for imaging. Furthermore, due to the available monochromaticity of up to 10000 spectromicroscopy of nano-sized particles were performed with this full-field X-ray microscope. For creating NEXAFS spectra image stacks with about 10 μm x 10 μm field of view containing a lot of nano-particles for statistics in less than 2 hours were accomplished. The setup and characteristics of the x-ray microscope are described. Results in the field of biology and material sciences are presented.

Reference: [1] Schneider et al., Nat. Methods 7 (2010), 985-987