Dresden 2011 – wissenschaftliches Programm
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Q: Fachverband Quantenoptik und Photonik
Q 22: Ultrakurze Laserpulse: Anwendungen 1
Q 22.5: Vortrag
Dienstag, 15. März 2011, 11:30–11:45, SCH A01
Superresolved femtosecond nanosurgery of cells — •Matthias Pospiech1, Moritz Emons1, Kai Kütemeyer2, Alexander Heisterkamp2, and Uwe Morgner1,2 — 1Leibniz Universität Hannover — 2Laserzentrum Hannover e.V.
We report on femtosecond nanosurgery of fluorescent labeled structures in cells with a spatially superresolved laser beam. The focal spot width is reduced below the diffraction limit using phase filtering applied with a programmable phase modulator. These superresolved focal spots are analyzed theoretically and experimentally. Cutting of cell structures is performed within an inverted Microscope and high NA Objectives. A comprehensive statistical analysis of the resulting cuts is presented, which demonstrates an achievable average resolution enhancement of 30 %