Berlin 2012 – scientific programme
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BP: Fachverband Biologische Physik
BP 1: Proteins I
BP 1.2: Talk
Monday, March 26, 2012, 10:00–10:15, H 1058
Temperature-induced denaturation of protein layers at solid-liquid interfaces - an x-ray reflectivity study — •Irena Kiesel1, Michael Paulus1, Julia Nase1, Sebastian Tiemeyer1, Christian Sternemann1, Anne K. Hüsecken2, Steffen Bieder1, and Metin Tolan1 — 1Fakultät Physik/DELTA, TU Dortmund, Maria-Goeppert-Mayer-Str. 2, 44227 Dortmund, Germany — 2Naturwissenschaftlich Technische Fakultät, Fachbereich Physik, Universität Siegen, Walter-Flex-Str. 3, 57068 Siegen, Germany
Protein adsorption at solid-liquid interfaces is crucial for many applications such as in food industry and medical devices. As proteins loose their functionality during denaturation, the state of the adsorbed proteins influence the growth of biofilms (e.g. bacteria) and the acceptance of implants in human body (e.g. growth of cells). Our aim is to understand the denaturation process of proteins at the solid-liquid interface. Until now, most denaturation processes were analysed in protein solutions, which serves as a protein reservoir. We investigate the protein denaturation process in two different environments by heating adsorbed protein layers up to 90° C and analyse them at each temperature step by x-ray reflectivity measurements. Therefor we use the 27 keV x-ray reflectivity set-up at BL9 at the synchrotron light source DELTA, Dortmund. The denaturation process is investigated by analyzing the (layer) electron density profile and with this, information on structural changes in the protein film induced by temperature are obtained.