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Berlin 2012 – scientific programme

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BP: Fachverband Biologische Physik

BP 1: Proteins I

BP 1.4: Talk

Monday, March 26, 2012, 10:30–10:45, H 1058

Sequence Independent DNA-to-DNA Binding at a Gold Surface Mediated by a Dimeric Protein — •Tihomir Solomun1, Alexander Kovalev1, Robert Wild2, Harald Seitz2, and Heinz Sturm1,31Federal Institute for Materials Research and Testing, Unter den Eichen 87, D-12205 Berlin, Germany — 2Fraunhofer Institut für Biomedizinische Technik, Am Mühlenberg 13, D-14476 Potsdam-Golm, Germany — 3Technical University Berlin, Pascalstrasse 8-9, D-10587 Berlin, Germany

Confocal fluorescence, surface plasmon resonance (SPR) and atomic force microscopy (AFM) methods were used to study the interaction of a dimeric gene-5-protein (g5p) with an adlayer of single-stranded DNA (ssDNA) oligonucleotides tethered to a gold surface. The data show that a highly stable g5p-ssDNA surface complex is readily formed. The extent of the complexion indicates involvement of cooperative protein-protein interactions. In the case where oligonucleotides are also present in the solution, sequence independent binding takes place between the non-complementary oligonucleotides in the solution and those immobilized on the surface. This binding is rendered possible by dimeric nature of the g5p protein.

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