Berlin 2012 – scientific programme

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BP: Fachverband Biologische Physik

BP 10: Biopolymers and Biomaterials (with CPP)

BP 10.5: Talk

Tuesday, March 27, 2012, 10:45–11:00, H 1058

Study of lipid and protein coatings on titanium surfaces by neutron scattering — •Maksym Golub¹, Regine Willumeit¹, Berengere Luthringer¹, Frank Feyerabend¹, Eric Watkins², Dieter Lott¹, Vasyl Haramus¹, Boris Toperveg³, and Andreas Schreyer¹ — ¹Helmholtz Zentrum Geesthacht, Geesthacht, Germany — ²ILL, Grenoble, France — ³Ruhr-University Bochum, Germany

Permanent implants, e.g. using titanium and its alloy, are widely used and successfully implemented in medicine. To improve their performance lipid*s covering is applied. The study of the structure of the phospholipid (palmitoyl-oleoyl-sn-glycero-3-phosphoethanolamine - POPE) layering under liquid conditions in presence of growth medium and Human Serum Albumin (HSA), which is the favourable condition for cell adhesion, would provide key parameters to understand the interaction between cells and lipid coated implants. Such a system was measured by neutron reflectivity. Silicon crystals with a Ti layer (36 nm) on the top were covered by POPE lipids and measured at different conditions: D2O, D2O based growth medium and growth medium with protein i.e., HSA. A 2D detector was used for the data collection which allowed us to detect also the diffuse scattering which provides information about the lateral correlations in these films. The neutron reflectivity experiments enabled us to see the changes of layer structure due to adhesion of the protein and will be discussed here in detail.