Berlin 2012 – scientific programme
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BP: Fachverband Biologische Physik
BP 17: Posters: Physics of Cells
BP 17.10: Poster
Wednesday, March 28, 2012, 17:30–19:30, Poster A
Cell migration on different substrates - An investigation with optical microscopy under homogeneous conditions — •Thorsten Robel1, Daniele Martini1, Michael Beil2, and Othmar Marti1 — 1Institute for Experimental Physics, Ulm University, 89081 Ulm, Germany — 2Internal Medicine I,Ulm University, 89081 Ulm, Germany
In this poster we discuss the influence of the substrate interaction of pancreatic carcinoma cells (line Panc-1).
The physical and chemical properties of different substrates can influence the cell motility. Over night time lapse video microscopy observations of cultivated cells were used to measure the Mean Square Displacement of the cells as well as their mean velocity. The set-up was based on an inverted Leica microscope (Leica Dmirb). An incubation chamber was used to regulate temperature, CO2-concentration and humidity to assure identical environmental conditions for the cells. From time lapse images the trajectories of a subset of all cells were extracted with the program ImageJ.
First measurements to compare the motility of Panc-1 cells with different other cell lines were accomplished.
These observations revealed different motilities regarding cell type and substrate.